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Thursday, May 2, 2019
Rothera's test Procedure - Test Principle, Uses, Preparation and Interpretation - Ketone Bodies
rothera's test procedure, Principle, Uses, reagents Preparation and results Interpretation with normal reference range - test for ketone bodies.
Principle of Rothera’s Test
Acetoacetic acid and acetone react with alkaline solution of sodium nitroprusside to form a purple colored complex. This method can detect above 1-5 mg/dl of acetoacetic acid and 10-20 mg/dl of acetone. Beta-hydroxybutyrate is not detected.
What is Rothera's test?
Why do ketone levels rise in plasma and urine?
Is Ketonuria a sign of dehydration?
Can you have ketones without having diabetes?
More Information:
http://laboratorytests.org/rotheras-test/
Credits:
Labs for life, a partnership project of MoHFW and CDC
https://www.youtube.com/channel/UCGCm7p6yg20dH3pJ8yzPNTQ
Westergren test Procedure - Test Principle, Uses, Preparation and Interpretation - ESR test
westergren Test procedure, Principle, Uses, reagents Preparation and results Interpretation of westergren method with normal reference range - ESR test.
A method for estimating the sedimentation rate of red blood cells in whole blood by mixing venous blood with an aqueous solution of sodium citrate and allowing the mixture to stand in an upright standard pipet and, after one hour, reading the millimeters the cells have descended.
What is ESR Westergren method?
What does high ESR mean?
What is Westergren tube?
What is a normal sed rate?
More Information:
http://www.medicine.mcgill.ca/physio/vlab/bloodlab/esr.htm
Credits:
Labs for life, a partnership project of MoHFW and CDC
https://www.youtube.com/channel/UCGCm7p6yg20dH3pJ8yzPNTQ
ELISA test Procedure - Test Principle, Uses, Preparation and Interpretation - HIV AIDS test
ELISA Test procedure, Principle, Uses, reagents Preparation and results Interpretation with normal reference range -HIV AIDS test.
The Enzyme-Linked Immunosorbent Assay (ELISA) is a technique used to detect antibodies or infectious agents in a sample. Antibodies are made in response to infection and so an antibody ELISA can indicate whether or not an animal has been in contact with a certain virus.
More Information:
https://en.wikipedia.org/wiki/ELISA
Credits:
Labs for life, a partnership project of MoHFW and CDC
https://www.youtube.com/channel/UCGCm7p6yg20dH3pJ8yzPNTQ
Wintrobe's method Procedure - Test Principle, Uses, Preparation and Interpretation - ESR test
wintrobe's method procedure, Principle, Uses, reagents Preparation and results Interpretation with normal reference range-ESR test.
Wintrobe method: The Wintrobe method is performed similarly except that the Wintrobe tube is smaller in diameter than the Westergren tube and only 100 mm long. EDTA anticoagulated blood without extra diluent is drawn into the tube, and the rate of fall of red blood cells is measured in millimeters after 1 hour.
More Information:
http://www.medicine.mcgill.ca/physio/vlab/bloodlab/esr.htm
Credits:
Labs for life, a partnership project of MoHFW and CDC
https://www.youtube.com/channel/UCGCm7p6yg20dH3pJ8yzPNTQ
Hay's test Procedure - Test Principle, Uses, Preparation and Interpretation - Bile salts test
hay's test procedure, Principle, Uses, reagents Preparation and results Interpretation with normal reference range bile salts test.
Hay's test, also known as Hay's sulphur flower test, is a chemical test used for detecting the presence of bile salts in urine.
Sulphur powder is sprinkled into a test tube with three millilitres of urine and if the test is positive, the sulphur powder sinks to the bottom of the test tube. Sulphur powder sinks because bile salts reduce the surface tension of urine.
More Information:
https://en.wikipedia.org/wiki/Hay's_test
Credits:
Labs for life, a partnership project of MoHFW and CDC
https://www.youtube.com/channel/UCGCm7p6yg20dH3pJ8yzPNTQ
Rapid Plasma Reagin Test Procedure - Test Principle, Uses and Interpretation - RPR test
Rapid Plasma Reagin Test procedure for syphilis diagnosis, Principle, Uses, reagents Preparation and results Interpretation with normal reference range RPR test.
cytochrome oxidase test Prodecure - Test Principle, Uses, Preparation and Interpretation
cyrochrome oxidase Test procedure, Principle, Uses, reagents Preparation and results Interpretation with normal reference range.
Purpose of Oxidase test
Oxidase test is most helpful in screening colonies suspected of being one of the Enterobacteriaceae (all negative) and in identifying colonies suspected of belonging to other genera such as Aeromonas, Pseudomonas, Neisseria, Campylobacter, and Pasteurella (positive).
Test requirements for Oxidase test: Moist filter paper with the substrate (1% tetramethyl-p-phenylenediamine dihydrochloride), or commercially prepared paper disk, wooden wire or platinum wire.
More Information:
https://en.wikipedia.org/wiki/Oxidase_test
Credits:
Labs for life, a partnership project of MoHFW and CDC
https://www.youtube.com/channel/UCGCm7p6yg20dH3pJ8yzPNTQ
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